Screening greater than 10 pathogens or SNPs in a sample is challenging due to limitations in fluorescence colors in a multiplex quantitative PCR. Optimizing a multiplex qPCR is daunting, and it limits ...

RT-qPCR stands for quantitative reverse transcription polymerase chain reaction, the “RT” not to be mistaken for “real time”. 2 Unlike qPCR that uses a DNA template, the starting material for RT-qPCR ...

According to a recent report on the gene-amplification technologies market from Global Industry Analysts, there are close to 70,000 bioresearchers using real-time quantitative PCR (qPCR) in North ...

qPCR is a potent technique for microRNA expression profiling to assess gene regulation, yet the characteristics of miRNAs present challenges for assay design. miRNAs are only 19–22 nucleotides in ...

From the start of the COVID-19 pandemic, diagnostic testing has been highlighted as a key part of global measures to contain the spread of SARS-CoV-2. In a media briefing on March 16, 2020, Dr Tedros ...

Multiplex qPCR is a popular area of interest for many assay developers. Thanks to distinct reporter dyes, multiple reactions can be combined into a single well, with each target detected independently ...

Leading PCR and qPCR technology now available for high-throughput applications: With the Biometra TRobot II and qTOWER³ auto, Analytik Jena presents two new thermal cyclers for fully automated PCR and ...

When working in a lab, launching a quantitative PCR (qPCR) experiment demands a comprehensive understanding of complicated protocols, principles, and methodologies. Analyzing the large amounts of ...