Single-stranded or double-stranded DNA junctions with recessed 5′ ends serve as loading sites for the checkpoint clamp, 9-1-1, which mediates activation of the apical checkpoint kinase, ATRMec1.

The clamp then closes and signals to the polymerases that they can begin replicating DNA. Using high-powered cryo-electron microscopes, Li, O'Donnell and their teams revealed previously unknown facets ...

The process of DNA replication relies on the coordinated action of a ring‐shaped sliding clamp and its specialised loader complexes. Sliding clamps, typified by proliferating cell nuclear antigen ...

Researchers in the lab of John Kuriyan, professor of biochemistry and dean of the School of Medicine Basic Sciences, have revealed a key element of how a molecular machine responsible for high-speed ...

Newswise — Every time a cell divides, it must copy its entire genome so that each daughter cell inherits a complete set of DNA. During that process, enzymes known as polymerases race along the DNA to ...

The clamp then closes and signals to the polymerases that they can begin replicating DNA. Using high-powered cryo-electron microscopes, Li, O'Donnell and their teams revealed previously unknown facets ...

Though scientists have learned much about DNA damage and repair over the past 50 years, important outstanding questions remain. One particularly bedeviling puzzle is how a repair protein called the ...

Humans and baker's yeast have more in common than meets the eye, including an important mechanism that helps ensure DNA is copied correctly, reports a pair of studies. The findings visualize for the ...

Scientists have discovered that a protein once thought to simply help load a factor necessary for the copying of DNA, actually plays a key role in ensuring fast and reliable replication—an insight ...

DNA replication is a complex process with many moving parts. In baker's yeast, the molecular complex Ctf18-RFC keeps parts of the replication machinery from falling off the DNA strand. Human cells use ...