The CRISPR-Cas system is by itself, and in combination with other technologies such as aptamers, isothermal amplification, and nanomaterials, being widely used in cancer diagnostic platforms.

Rather than being famous in gene editing field only, by revealing the collateral cleavage activity of Cas12a, Cas13a, Cas14 effectors, and more, Clustered Regularly Interspaced Short Palindromic ...

“Instead of just one gene – as is the case with other CRISPR systems like the more familiar Type II Cas-9 system – we can bring in a whole metabolic pathway to incorporate a whole new set of ...

Key Points: 1. CRISPR/Cas as a Diagnostic Tool The CRISPR/Cas system, known for gene editing, is now revolutionizing molecular diagnostics due to its high specificity and efficiency in recognizing ...

CRISPR-Cas9, the genome-editing approach that won the Nobel Prize in Chemistry in 2020, was adapted from a naturally occurring genome editing system bacteria use as a defense mechanism. CRISPR-Cas ...

Employing a metagenomic search, we identified a family of miniature CRISPR-Cas type V-L systems capable of RNA-guided dsDNA target cleavage without a tracrRNA. A bacterial depletion screen revealed ...

The CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) and its associated protein (Cas9) system is a genome-editing technology that holds the potential to alleviate ...

The researchers found the Fanzor system to initially be less efficient at snipping DNA than CRISPR/Cas systems, but by systematic engineering, they introduced a combination of mutations into the ...

The researchers found the Fanzor system to initially be less efficient at snipping DNA than CRISPR/Cas systems, but genetically engineered the protein to increase its activity 10-fold.

Finally, we developed a novel ternary vector system to integrate the MR and CRISPR/Cas modules. Our ternary vector system is composed of new pGreen-like binary vectors, here named pGreen3, and a ...